Hct116 dko cells
WebSome of these miRNA have been known to be methylated in a cancer-specific manner. In the colorectal cancer HCT-116 cell line, about 6% of the 320 analyzed miRNAs were up … WebThis product is genomic DNA purifiedfrom DNMT DKO (double knock-out) human HCT116 cell line, which is genetically lacking both of methyltransferases DNMT1 (DNA …
Hct116 dko cells
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WebCpGenome™ Human Methylated & Non-Methylated DNA Standard Set Purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 ( … WebHCT116 and DKO Whole Genome Bisulfite sequencing data (hg19) from (Blattler et al., 2014) were lifted over to hg38 using Crossmap (Zhao et al., 2014). DNA methylation tracks for HCT116 and 5Aza ...
HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. Furthermore, it was found that the knockout of MARCH2 limited … WebMay 16, 2024 · Human colorectal cancer cells HCT116 and DKO cells were cultured in 1 g/L glucose DMEM supplemented with 10% FBS and 1 × NEAA (Thermo Scientific, Loughborough, UK). Human neuroblastoma SHSY5Y cells were cultured in DMEM/F12 medium supplemented with 10% FBS (Thermo Scientific) and treated with Aza (Sigma …
WebAnti-HSP27 antibody was purchased from Abcam (Cambridge, UK). HCT116, HT-29 and DLD-1 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The cells were cultured in RPMI with 10% fetal bovine serum (FBS) in a humidified incubator (37 °C, 5% CO 2). The cells were either sub-cultured or used … WebAug 19, 2014 · The human colorectal carcinoma cell lines: HCT116, SW480, Colo320, and RKO, which were obtained from the American Type Culture Collection (ATCC, VA, USA), …
WebJun 29, 2024 · HCT116 wild-type and HCT116 DKO scRNA libraries based on the 10× Genomics platform were generated using the Chromium Single Cell 3′ Library & Gel …
WebJun 15, 2024 · HCT116, DKO and 293 T cells were cultured in DMEM/High Glucose media (Caisson labs) according to manufacturer’s instructions, supplemented with 10% Fetal … ft to in 2WebTo test this hypothesis, we induced acute hypomethylation in HCT116 cells by 5-aza-2'-deoxycytidine (5-aza-CdR) treatment (HCT116-5-aza-CdR) and compared that to DKO … ft to in poundsWebApr 11, 2024 · This finding was supported by the fact that USP4 overexpression in HCT116 cells with reduced expression of CTTN was insufficient to promote cell migration. Additionally, we observed that USP4 overexpression led to a significant increase in CTTN phosphorylation, which is a requisite mechanism for cell migration, by regulating … ft to knWebDec 14, 2024 · We used the CRISPR-Cas9 system to sequentially delete both Mediator kinase paralogs, CDK8 and CDK19, in two CRC lines exhibiting CDK8/19 copy number disomy and relative average expression level (HCT116 and DLD1) ( Firestein et al., 2008 ). These lines will be referred to as double-knockout (DKO) lines ( Figure S1 A). ft to jodWebMar 25, 2024 · HCT116 and DKO cells were cultured in McCoy5A medium. DKO cells were grown in the presence of G418, geneticin. All media were supplemented with 10% FBS at 37 °C and 5% CO 2. For drug treatment ... ft to in to cmWebMay 15, 2024 · cell line: HCT116 colon cancer cells genotype/variation: DKO (expressing hypomorphic DNMT1 and lack DNMT3b) medip antibody: 5-mC (Diagenode, catalog# … ft to in tableWebAug 19, 2014 · The human colorectal carcinoma cell lines: HCT116, SW480, Colo320, and RKO, which were obtained from the American Type Culture Collection (ATCC, VA, USA), as well as a double-knockout cells (DKO) for DNA methyltransferase-1 and DNA methyltransferase-3b in HCT116 cell line [16], which retains <5% genomic DNA … ft to inc